Extended Data Fig. 1: Serine and glycine metabolism in diabetes.
From: Insulin-regulated serine and lipid metabolism drive peripheral neuropathy
(a) Levels of glycine, serine, and methionine in WT and BKS-db/db mice in the kidney (n = 6 per group). (b) Levels of glycine, serine, and methionine in WT and BKS-db/db mice in the inguinal adipose tissue (iWAT) (n = 6 per group). (c) Plasma metabolite levels in WT and BKS-db/db mice (n = 6 per group). (d) mRNA expression of kidney enzymes regulating serine, glycine, and one-carbon metabolism in WT (n = 6) and BKS-db/db mice (n = 5). (e) Serine tolerance test (STT) in C57BL/6J mice after an overnight fast (n = 4 per dose). (f) Tissue serine labeling fraction in WT mice 15 min after [U-13C3]serine administration via oral gavage (n = 4 per tissues) after an overnight fast. (g) Tissue citrate labeling fraction in WT mice 15 min after [U-13C3]serine administration via oral gavage (n = 4 per tissues) after an overnight fast. Data are mean ± standard error of mean (SEM) and were analyzed using a two-sided independent t-test (a–d). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. WT group.
