Extended Data Fig. 2: Serine tolerance test in type 1 and 2 diabetic mice.
From: Insulin-regulated serine and lipid metabolism drive peripheral neuropathy
(a) Serine dehydratase activity determined in the liver and kidney in C57BL/6J mice in the fed state (n = 5 per tissues). (b) Serum insulin in WT and BKS-db/db mice quantified after a 6-h fast (n = 6 per group). (c) Serum glucagon in WT (n = 5) and BKS-db/db (n = 6) mice quantified after a 6-h fast. (d) Plasma glucose pharmacokinetics and area under curve (AUCGLC) in WT and BKS-db/db mice following GTT/STT with glucose dosed at 2 g/kg (n = 6 per group) after an overnight fast. (e) Plasma glycine pharmacokinetics and area under curve (AUCGLY) in WT and BKS-db/db mice following GTT/STT with glucose dosed at 2 g/kg (n = 6 per group) after an overnight fast. (f) Serine tolerance test (STT) and area under curve (AUCSER) in WT and BKS-db/db mice (n = 6 per group) dosed with serine (400 mg/kg) only after an overnight fast. (g) Fasting (6-h) plasma insulin level in C57BL/6J mice treated with vehicle or streptozotocin (STZ) 1 week after injection (n = 9 per group). (h) Fasting (6-h) blood glucose in C57BL/6J mice treated with vehicle or streptozotocin (STZ) 1 week after injection (n = 9 per group). (i) Body fat mass content in C57BL/6J mice treated with vehicle or streptozotocin (STZ) 1 week after injection (n = 9 per group). (j) Fasting (6-h) plasma amino acid concentration in C57BL/6J mice treated with vehicle (n = 9) or streptozotocin (STZ, n = 10) 1 week after injection. (k) Plasma glucose pharmacokinetics and area under curve (AUCGLC) after an overnight fast during GTT/STT in C57BL/6J mice treated with vehicle (n = 7) or streptozotocin (STZ, n = 6) 2 weeks after injections with glucose dosed at 2 g/kg. (l) Thermal latency in 14-week-old WT and BKS-db/db mice (n = 6 per group). (m) Tactile sensing in 14-week-old WT and BKS-db/db mice (n = 6 per group). (n) Motor nerve conduction velocity in 14-week-old WT and BKS-db/db mice (n = 6 per group). Data are mean ± standard error of mean (SEM) and were analyzed using a two-sided independent t-test (b–n) and a two-way ANOVA with Fisher’s LSD post hoc test (e–f, k time-course experiments).
