Fig. 1: SRK controls stigmatic ROS in SI to reject intraspecific and interspecific pollen.
From: Stigma receptors control intraspecies and interspecies barriers in Brassicaceae
a, Open flowers and phylogenetic tree for Brassicaceae species from maximum likelihood analysis using internal transcribed spacer sequences. Distantly related Cochlearia danica served as an outgroup. Scale bar, mean number of nucleotide substitutions per site. b, Aniline blue staining showing intraspecific and interspecific pollen growth in mature and bud-stage B. rapa stigmas. c, H2DCFDA staining of ROS in unpollinated (UP) or pollinated B. rapa stigmas. d,e, Relaxed SI and UI in AS-BrSRK46-treated B. rapa S46 stigmas (d) and stigmas from SRK-defective B. rapa, BrSRK∆TM (e). f,g, Intraspecific and interspecific pollen (f) and ROS responses (g) in stigmas from SC A. thaliana, that is, wild type (WT), and stigmas from SI A. thaliana expressing A. halleri S13 genes (A. tha-S13). The values in the b–g images, shown as average ± s.d., indicate average number of pollen tubes in the stigma (b,d,e,f) and average ROS intensity (c,g). The same data are also presented in box plots with all data points (Extended Data Figs. 1, 2). Scale bars, 0.5 cm (a), 500 μm (b–e) and 200 μm (f,g). Each experiment was repeated at least three times with consistent results.
