Extended Data Fig. 10: Breaking interspecific barriers in Brassica crops.
From: Stigma receptors control intraspecies and interspecies barriers in Brassicaceae
a, b, Reducing ROS by Na-SA or increasing NO by GSNO increased the number of enlarged ovules in the pod of ♀B. rapa × ♂B. oleracea or ♀B. rapa × ♂B. vulgaris. See Fig. 5a. c, Suppressing the expression of BrSRK46, BrBrFER1, or BrRBOHF by AS-ODNs increased the number of enlarged ovules in the pod of ♀B. rapa × ♂B. oleracea or ♀B. rapa × ♂B. vulgaris. See also Fig. 5b. d, B. oleracea pollen tubes or B. vulgaris pollen tubes in B. rapa pistils. The reduced number of enlarged ♀B. rapa × ♂B. vulgaris ovules is possibly due to fewer B. vulgaris pollen tubes had exited the transmitting tissue and targeted the B. rapa ovules due to additional barriers based on their evolutionary distance (Fig. 1a). Arrowheads point to bundles of pollen tubes in the transmitting tissue, stars point to ovules penetrated by a pollen tube. Scale bars, 100 μm (d). Box plots (a–c): centre line, median; box limits, lower and upper quartiles; dots, individual data points; whiskers, highest and lowest data points. n (in blue), number of pistils. P values, two-tailed t-tests. Each experiment was repeated at least thrice with consistent results.
