Extended Data Fig. 3: FER/ANJ signalling regulates stigmatic ROS during UI.
From: Stigma receptors control intraspecies and interspecies barriers in Brassicaceae
a, b, Quantitative RT-PCR showing AS-BrFER1 treatment suppressed the expression of BrFER1(a) and promoted the growth of SI pollen and B. oleracea pollen in mature B. rapa stigmas and B. vulgaris pollen in bud stage (b) B. rapa stigmas. See Fig. 2a. c, d, AS-BrFER1 treatment suppressed SI and UI-induced ROS increase in mature and bud stage B. rapa stigmas at 10 MAP. e, f, The expression of FER and SRK in stigmas of A. tha (SI)/fer-4 (e) and loss of FER in SI A. thaliana promoted the growth of SI pollen, B. oleracea pollen and B. vulgaris pollen in SI A. thaliana stigmas (f). See Fig. 2b. g, Loss of FER in SI A. thaliana suppressed SI and UI-induced ROS increase in A. tha (SI)/fer-4 stigmas at 10 MAP. h, Domain structures of BrANJs proteins showing only BrANJ1 have intact extracellular and intracellular domain. i, j, AS-BrANJ1 treatment suppressed the expression of BrANJ1(i) and promoted the growth of SI pollen and B. oleracea pollen in mature B. rapa stigmas and B. vulgaris pollen in bud stage B. rapa stigmas (j). k, l, AS-BrANJ1 treatment suppressed SI and UI-induced ROS increase in mature (k) and bud stage (l) B. rapa stigmas at 10 MAP. Scale bars, Scale bars, 500 μm (c, d, j, k, l), 200 μm (g). Data bars (a, e, i): average ± SD. Average relative expression levels from three biological replicates of stigmas (two tailed t-test, n = 3). Box plots (b–d, f, g, j–l): centre line, median; box limits, lower and upper quartiles; dots, individual data points; whiskers, highest and lowest data points. n (in blue), number of stigmas. P values, two-tailed t-tests. Each experiment was repeated at least thrice with consistent results.
