Extended Data Fig. 4: ROS are produced by RBOHs during UI.
From: Stigma receptors control intraspecies and interspecies barriers in Brassicaceae
a, Quantitative RT-PCR showing AS-BrRBOHF treatment suppressed the expression of BrRBOHF. b, c, AS-BrRBOHF treatment suppressed SI and UI-induced ROS increase in mature (b) and bud-stage (c) B. rapa stigmas at 10 MAP. d, The RBOH enzymatic activity in mature B. rapa stigmas were increased after pollination with SI and UI pollen but decreased after pollination with CP pollen. e, AS-BrRBOHF treatment promoted the growth of SI pollen and B. oleracea pollen in mature and B. vulgaris pollen in bud-stage B. rapa stigmas. Scale bars, 500 μm. Data bar (a): average ± SD. Average relative expression levels from three biological replicates of stigmas (two tailed t-test, n = 3). Data bars (d): Average activity of ROS producing enzymes from three technical replicates of one stigma sample containing 100 stigmas (two tailed t-test, n = 3). Box plots (b, c, e): centre line, median; box limits, lower and upper quartiles; dots, individual data points; whiskers, highest and lowest data points. n (in blue), number of stigmas. P values, two-tailed t-tests. Each experiment was repeated at least thrice with consistent results unless otherwise specified.
