Extended Data Fig. 6: Deletion of the GATA site ablates binding of PROX1, FOXC2 and NFATC1 to the Prox1 â11âkb enhancer.
From: A Prox1 enhancer represses haematopoiesis in the lymphatic vasculature
a, ChIP assays using primary LECs isolated from mutant embryos at E17.5 show no enrichment over IgG control when the GATA2 site is absent. b, Model proposing that GATA2 acts as a pivotal factor at the Prox1 â11âkb enhancer to promote recruitment of transcriptional componentry responsible for driving Prox1 expression in LECs. c, Quantification of interaction frequency of the Prox1 â11âkb enhancer with the Prox1 promoter in cultured human LEC. Chromosome Conformation and Capture (3C) analysis of regions proximal to the Prox1 gene demonstrates increased interaction frequency relative to a BAC control, of the anchor fragment (containing the promoter) and fragments X (containing the enhancer) and XII. Data are shown as mean values Âą SD, nâ=â3. Primer sequences and quantification are available in Source Data Extended Data Fig. 6.
