Extended Data Fig. 5: Transcriptomic analysis of lymphatic endothelial cells indicates a shift in identity of enhancer mutant LECs towards that of blood endothelial cells.
From: A Prox1 enhancer represses haematopoiesis in the lymphatic vasculature
a, Differential gene expression in RNA-seq analysis of LECs isolated from mutant and wildtype embryos at E14.5. Selected genes are ranked in order of expression level in wildtype (highest to lowest, left to right) with markers of LEC identity (red) and BEC identity (blue) highlighted. b, Heatmap comparing microarray analysis of E14.5 LEC and BEC RNA with RNA-seq data shows genes up-regulated in enhancer mutant LECs correlate with genes expressed at higher levels in BECs and vice versa. Green bars indicate genes with a positive correlation to a shift in identity of LEC to BEC in mutant versus wildtype. A Fisher’s Exact Test shows the association between microarray and RNA-seq outcomes is significant, two-tailed p value < 0.0001.
