Extended Data Fig. 7: scRNA-seq of the Segmentoid and the Somitoid model.
From: Reconstruction and deconstruction of human somitogenesis in vitro
a, UMAP embedding of cells merged from both models (19,551 cells) coloured with cell types identified with Leiden clustering. b, Dot plot of selected genes in cell type clusters from both models. c, Machine-learning classification of a previous data set of E9.5 mouse embryo. d, e, Classifier analysis on cell types comparing the in vitro models with mouse E9.5. A k-NN classifier trained on clusters of mouse clusters was used to predict identities of the human in vitro models. f, g, Classifier analysis on cell types (f) and time points (g) comparing Somitoids with Segmentoids. h, Mean expression heatmap of selected genes in the three datasets. i, Top, somite sub-cluster highlighting cells expressing TBX18 (left) and UNCX (right); Bottom, number of cells expressing TBX18, UNCX, or both in Segmentoids (left) and Somitoids (right). j, k, l, Dot plots of HOX-family genes expression at various timepoints of the Segmentoid model (j), the Somitoid model (k), and the NMP cells of the Segmentoid (l). The mean expression of each cluster is scaled per gene.
