Extended Data Fig. 8: Antero-Posterior patterning and the segmentation clock.
From: Reconstruction and deconstruction of human somitogenesis in vitro
a, b, Time auto-correlation of HES7 (a) and MESP2 (b) reporter oscillations in individual WT Segmentoids. Triangles indicate auto-correlation peaks, which in turn indicate oscillation period. c, Merged maximum-z-projection confocal image of a Segmentoid with UNCX reporter, DAPI, and Phalloidin staining (n>10 Segmentoids). d, Distribution of rosette numbers in each segment along the anterior-posterior axis. A segment is defined as the posterior boundary of the UNCX stripe to that of the next posterior UNCX stripe. The maximal number of rosettes along the AP axis observed was used to represent the entire segment (n = 24 Segmentoids). e, Distribution of rosette numbers in each segment along the medial-lateral axis (left; n = 25 Segmentoids). The data is re-grouped based on relative AP location in the Segmentoids (right). f, Kymographs of reporters for pseudoHES7, UNCX, and MESP2 in the same HES7-null Segmentoid. g, Wide-field images and graphs of reporter intensities from posterior (P) to anterior (A) end along 120 h HES7-null (left) and WT (right) Segmentoids (n>10 Segmentoids for each condition). h, Time-lapse, maximum-z-projection confocal images of MESP2 reporter in a HES7-null Segmentoid. i, Average nematic order of MESP2/UNCX signals in WT and HES7-null Segmentoids as a function of time (mean±s.d; n = 7 WT Segmentoids and n = 6 HES7-null Segmentoids). Statistics was performed with a Wilcoxon rank-sum test (two-sided) and P-value is shown. j, Summary of HES7-null phenotypes in Somitoid and Segmentoid. All scale bars represent 100 µm.
