Extended Data Fig. 1: Characterization of the Somitoid model.
From: Reconstruction and deconstruction of human somitogenesis in vitro
a, Time lapse confocal images of H2B-mCherry in a spreading Somitoid. b, Illustration of the design of the HES7/MESP2 double-reporter cell line. c, Left, time-lapse confocal images of HES7 wave; Right, temporal profiles of HES7 reporter in two different regions indicated by the blue and orange boxes. d, Left, box plots of projected areas of all rosettes in individual Somitoids. Right, plot of median rosette area of each Somitoid (n = 20 Somitoids). Red bars indicate median with interquartile range. e, Correlation analysis (n = 20 Somitoids; two-sided) between the entire Somitoid area and median rosette area (left); between the entire Somitoid area and total rosette number (right). f, Shape descriptors of individual rosettes (top) and entire Somitoids (bottom). n = 1,957 rosettes from 20 Somitoids. g, h, Confocal slices from the bottom (z = 0 µm) to the top of a rosette in 120 h Somitoid stained with Laminin (g) and N-Cadherin (h) (n = 2 Somitoids). i, Representative images of a Somitoid cultured on gelatin (n = 5 Somitoids) or laminin (n = 5 Somitoids) coated surface, stained with Laminin. j, 3D reconstruction image of a Somitoid cultured in suspension (left; n = 2 Somitoids) and a confocal section (right), stained with Laminin. k, Principal components analysis using the same RNA sequencing datasets shown in Fig. 1h. l, Confocal images of 120 h PAX3-reporting Somitoids treated with 5 µM Blebbistatin (left) and control (right). In box-and-whiskers plots, the middle hinge corresponds to median, the lower and upper hinges correspond to the first and third quartiles, respectively, and the lower and upper whiskers correspond to the minimum and maximum, respectively. Scale bars represent 500 µm (a, c, i, l), 50 µm (g, h) and 100 µm (j).
