Extended Data Fig. 5: Expression of TBXT and SOX2 in Segmentoids.
From: Reconstruction and deconstruction of human somitogenesis in vitro
Confocal images of immunostaining of TBXT and SOX2 at 24 h (a), 48 h (b), 72 h (c), 96 h (d), and 120 h (e) of the Segmentoid model. Representative maximum-z-projection images are shown from b-e. 48 h, n = 3 Segmentoids; 72 h, n = 11 Segmentoids; 96 h, n = 17 Segmentoids; 120 h, n = 21 Segmentoids, with 7 Segmentoids still showing apparent TBXT and SOX2 double positive pole. A, anterior; P, posterior. Scale bars (a, b) represent 100 µm and 20 µm in corresponding enlarged views; Scale bars (c, d, e) represent 100 µm.
