Extended Data Fig. 5: Structure determination: NusG-Rho-TEC (n = 6, 7, and 8).
From: Structural basis of Rho-dependent transcription termination
(a) Data processing scheme (n = 8). (b) Representative electron microphotograph and 2D class averages (n = 8; 50 nm scale bar in right subpanel). (c) Orientation distribution (n = 8). (d) Fourier-shell correlation (FSC) plot. (e) EM density map coloured by local resolution (n = 8) (view orientation as in Fig. 2a, left). (f) EM density maps for NusG-Rho-TEC obtained using nucleic acid scaffolds with n = 6, 7, and 8 (view orientation as in Fig. 2a, left). (g-j) Representative EM density (blue mesh) and fits (ribbons) for RNAP regions that interact with Rho, NusG, Rho protomer C, spacer RNA, and SBS ligand.
