Extended Data Fig. 1: ALOX15, but not ALOX5, is required for AM homeostasis.
From: Neonatal imprinting of alveolar macrophages via neutrophil-derived 12-HETE
(a) AM frequencies in the lungs of adult WT (n = 7) and Alox15−/− (n = 6) mice. (b) Representative FACS plot and quantification of AM frequencies in the BAL of adult WT and Alox15−/− mice (n = 9 mice/group). (c) Representative FACS plot and quantification of AM frequencies and numbers in the lungs of adult Alox15−/− (n = 9) and Alox15+/+ (n = 7) littermate control mice. (d) Representative FACS plot and quantification of AM frequencies and numbers in the lungs of adult WT (n = 10) and Alox5−/− (n = 9) mice. (e) Quantification of lung innate cells in adult WT and Alox15−/− mice (n = 8/8/8/8/6/6/6/group). See also Supplementary Fig. 1 for gating strategy. (f) Weight of spleen, lungs, brain and top liver lobe from WT and Alox15−/− adult mice (n = 8 mice/group). (g-h) Representative FACS plot (g) and quantification (h) of resident tissue macrophages in adult WT (n = 3/6/7/7/group) and Alox15−/− mice (n = 3/5/6/6/group). (i) Bone marrow chimera model. (j-k) Representative FACS plots (j) and AM chimerism 8 weeks post-BM reconstitution (right) (n = 8/11/11/9/group). Data are presented as mean ± s.e.m and are pooled from two (a, c-e, f-k) or three (b-e) independent experiments and were analyzed using unpaired two-tailed t-test (a, c) or two-way ANOVA followed by Sidak’s multiple comparisons test (k). The model in (i) was created using BioRender (https://biorender.com).
