Extended Data Fig. 8: Conservation of the CoV active site across clades and SARS-CoV-2 strains.
From: Structural basis for substrate selection by the SARS-CoV-2 replicase
(a) Zoom-in on the active site of S4_GTP, highlighting residues (sticks) which interact with the bound GTP/2xMg in which ribbon/sticks are colored according to the amino acid conservation across a representative list of viruses found in the α & β coronavirus clades (Supplementary Table 3). (b) Bar plot showing the frequency of occurrence of the wild-type amino acid (reference strain Wuhan/Hu-1/2019) as well as their summed mutations according to the GISAID database, as of April 2022, for the nsp12 active site residues and the nsp12 residue P323. (c) Bar plot showing the frequency of occurrence of the wild-type amino acid (reference strain Wuhan/Hu-1/2019) as well as their summed mutations according to the GISAID database, as of April 2022, for Spike (S) residues found in the ACE2 binding region.
