Extended Data Fig. 2: Cryo-EM processing pipeline and analysis for S2_ATP dataset.
From: Structural basis for substrate selection by the SARS-CoV-2 replicase
(a) Cryo-EM processing pipeline for the S2_ATP dataset. (b) Angular distribution plot for the S2_ATP dataset, calculated in cryoSPARC. Scale depicts number of particles assigned to a specific angular bin. (c) Nominal 3.09 Å resolution cryo-EM reconstruction filtered by local resolution and colored according to fitted model chain. Right panel is clipped to reveal RTC active site. (d) Directional 3D FSC for S2_ATP, determined with 3DFSC. (e) Gold-standard FSC plot for the S2_ATP dataset, calculated by comparing two half maps from cryoSPARC. The blue dotted line represents the 0.143 FSC cutoff.
