Extended Data Fig. 6: Activation markers and single-cell phenotypes in lung adenocarcinoma.
From: Single-cell spatial landscapes of the lung tumour immune microenvironment
a, T-distributed stochastic neighbour embedding (t-SNE) of 108,387 endothelial cells. pSTAT3, Ki-67, CD39 and pERK expression within the endothelial cluster is shown. b, t-SNE plots of 9,480 mast cells, 42,427 neutrophils, 1,407 dendritic cells, 10,000 subsampled CD163- macrophages, 39,502 CD163+ macrophages, 37,653 classical monocytes, 8,330 intermediate monocytes and 17,029 non-classical monocytes. Ki-67, HIF1α, MMP9, ARG1, pERK, MCSFR, PD-1, B7-H3, BCL2, B7-H4, CD40, CC3, CD39, PD-L1 and pSTAT3 expression in the myeloid compartment is shown. c, t-SNE plots of 62,941 B cells, 98,396 Tc, 147,980 TH, 19,839 Treg and 23,995 T other cells. Ki-67, FOXP3, pERK, CD40, CD39, BCL2, PD-1 and pSTAT3 expression in the lymphoid compartment is shown. d-e, Prevalence of Ki-67+ endothelial cells (Comparison between Lepidic and Solid: * P = 0.0362. Comparison between Acinar and Solid: * P = 0.0185) and pERK+ TH cells (Comparison between Lepidic and Solid: **** P = <0.0001. Comparison between Lepidic and Micropapillary: *** P = 0.0004. Comparison between Lepidic and Acinar: *** P = 0.0003. Comparison between Lepidic and Papillary: * P = 0.0102. Comparison between Acinar and Solid: * P = 0.0288) as a proportion of endothelial and TH cells respectively, across histological subgroups in 416 lung adenocarcinoma patients (Lepidic n = 40, Papillary n = 33, Acinar n = 190, Micropapillary n = 35, Solid n = 118). Mean ± SEM. Statistical analysis (d-e: one-way ANOVA with Tukey multiple comparison test).
