Extended Data Fig. 9: The spatial cellular neighbourhoods of glioblastoma and brain metastases.
From: Single-cell spatial immune landscapes of primary and metastatic brain tumours
a, Representative Voronoi diagrams of CNs mapped onto glioblastoma IMC images. b, Representative Voronoi diagrams of CNs mapped onto BrM-core IMC images. c, Kaplan-Meier analysis of BrM patients (lung, melanoma and breast primaries) based on median CN5 frequency in BrM-cores. CN frequencies were averaged when multiple samples corresponded to the same patient. Log-rank (Mantel-Cox) test, n = 13 patients/group. d, CD45 expression in undefined cells across glioblastoma and BrM samples (n = 389 images). e, Distribution of CNs across BrM samples. BrM-margins: melanoma n = 6 images, lung n = 22 images, breast n = 12 images; BrM-cores: melanoma n = 13 images, lung n = 29 images, breast n = 17 images. For each image, the percentage of cells from each CN was determined, then averaged for each group. f, Schematic of the glioblastoma LTS/STS cohort and inclusion/exclusion criteria, pertaining to Supplementary Table 2. Created with BioRender.com. g, Kaplan-Meier analysis of glioblastoma LTS/STS cohort based on median CN frequency. CN frequencies were averaged when multiple samples corresponded to the same patient. Log-rank (Mantel-Cox) test, n = 16 patients/group.
