Extended Data Fig. 7: Telomere dysfunction is required for ZBP1-dependent IFN signalling.
From: Telomere-to-mitochondria signalling by ZBP1 mediates replicative crisis
a, Line chart showing growth curves analysis of IMR90 or WI38 expressing either empty vector (pLXSN3), HPV-E6E7 or SV40-LT in combination with an empty vector (pBABE), or hTERT. Cells with empty vector (pLXSN3) are indicated as CTR. One experiment was performed. b, TRF assays performed on IMR90 and WI38 expressing either HPV-E6E7 or SV40-LT in combination with an empty vector (pBABE) or hTERT. Two experiments were performed. DNA extracts were collected from cells at the indicated PDs. c, Scatter plots showing RT-qPCR analysis of ZBP1 levels in cells as in a. PDs are indicated. Expression levels were normalized to control cells with the earliest PDs. Bars represent mean ± s.d. from biological replicates. n: number of biological replicates. One-way ANOVA, ns: not significant, *** p < 0.001. Three independent experiments were performed. Abbreviations: hTERT: human telomerase reverse transcriptase; CTR: control; PDs: population doublings.
