Extended Data Fig. 2: Sucralose limits T cell proliferation without affecting viability.
From: The dietary sweetener sucralose is a negative modulator of T cell-mediated responses
a) CD4+ T cell proliferation assay performed with αCD3 (5 μg ml−1) and αCD28 (1 μg ml−1) for 3 days in the presence of serial dilutions of the indicated sweeteners. b) Proliferation of CFSE stained CD8+ T cells exposed to 0.5 mM of the indicated sweeteners and activated with a high dose of αCD3 (5 μg ml−1) or low dose of αCD3 (1 μg ml−1) in the presence or absence of αCD28 (1 μg ml−1). c) Jurkat T cell proliferation in control media or media supplemented with the indicated sweeteners. N = 3. d) Percentage of viable CD8+ (left) and CD4+ (right) T cells 24 h post activation with αCD3 (2 μg ml−1) and αCD28 (1 μg ml−1). Cell viability was determined by Fixable Viability Dye eFluor780® exclusion. Means represent ± s.e.m. for biological (d) or technical (c) replicates. Statistical significance was determined using one-way ANOVA with Tukey’s (c) or Dunnet’s (d) multiple comparison test. Data are representative of 3 independent experiments (c).
