Extended Data Fig. 6: Linearity of ΔF/F₀ of jGCaMP8, jGCaMP7, and XCaMP variants in cultured neurons.

Each gray dot represents a single well. ΔF/F₀ values in the 1–10 and 1–40 pulse range were fit to a linear model (orange and green, respectively). The slopes (m) and R² values are reported for each fit. jGCaMP8f, 29 wells, 594 neurons; jGCaMP8m, 16 wells, 408 neurons; jGCaMP8s, 12 wells, 121 neurons; GCaMP6s, 14 wells, 187 neurons; jGCaMP7s, 14 wells, 177 neurons; jGCaMP7c, 13 wells, 117 neurons; XCaMP-Gf, 14 wells, 194 neurons; 2 independent transfections, four 96-well plates. The jGCaMP8 sensors were moderately linear and exhibited a large slope in the 1–10 AP range (0.59 ≤ R² ≤ 0.82; 0.18 ≤ m ≤ 0.28), but less linear and exhibited a lower slope in the 1–40 AP range (0.43 ≤ R² ≤ 0.75; 0.052 ≤ m ≤ 0.081). On the other hand, GCaMP6s, jGCaMP7c, and XCaMP-Gf better maintained their linearity throughout the 1-40 AP range, but they had generally lower slopes in the 1–10 AP range (0.16 ≤ m ≤ 0.18).