Fig. 2: jGCaMP8 performance in Drosophila.
From: Fast and sensitive GCaMP calcium indicators for imaging neural populations
a, Schematic of the experiment. Fly with visual stimulus (top), fluorescence micrograph of L2 dendrites in medullar layer 2 (scale bar, 5 μm) (middle), and a schematic of the Drosophila visual system (bottom) are shown. b, ΔF/F0 response to a 0.5-Hz visual stimulation frequency from variants jGCaMP7f and jGCaMP8m. Individual traces show four representative individual animals per GECI (shading arbitrary). Light and dark periods are indicated by white and black bars above the graph. The error bands represent s.e.m. c, Mean ΔF/F0 response to 0.5-Hz stimulation. The solid line indicates the mean and the shaded area denotes s.e.m. The dark period is represented by a black bar above the graph. The mean was calculated from eight trials per animal and then between animals. The inset compares the response from each variant at the onset of the dark period. d, Half-rise and half-decay times for responses in c. Half-rise: 128 ± 11 ms (jGCaMP7f), 76 ± 8 ms (jGCaMP8f), 58 ± 6 ms (jGCaMP8m) and 80 ± 8 ms (jGCaMP8s) (Kruskal–Wallis multiple-comparison test, P = 2.9 × 10−4; pairwise Dunn’s comparison test with jGCaMP7f: P = 3.1 × 10−3 (jGCaMP8f), P = 2.9 × 10−5 (jGCaMP8m) and P = 1.3 × 10−2 (jGCaMP8s)). Half-decay times: 277 ± 29 ms (jGCaMP7f), 192 ± 26 ms (jGCaMP8f), 137 ± 21 ms (jGCaMP8m) and 198 ± 21 ms (jGCaMP8s) (Kruskal–Wallis multiple-comparison test, P = 2.4 × 10−2; pairwise Dunn’s comparison test: P = 1.1 × 10−1 (jGCaMP8f), P = 2.2 × 10−3 (jGCaMP8m) and P = 1.8 × 10−1 (jGCaMP8s)). *P < 0.05. Total n of flies tested for each variant in c and d: 14 (jGCaMP7f), 11 (jGCaMP8s), 11 (jGCaMP8m) and 14 (jGCaMP8f). For the box-and-whisker plots, the box indicates the median and 25–75th percentile range, and the whiskers indicate the shorter of 1.5 times the interquartile range or the extreme data point.
