Extended Data Fig. 16: Responses in fast-spiking interneurons.
From: Fast and sensitive GCaMP calcium indicators for imaging neural populations
a. Spike waveform parameters for each recorded cell; colors represent the expressed sensor, and the size of the circle represents average firing rate. Peak-to-trough ratios larger than 10 are plotted as 10. We defined putative interneurons as cells occupying the lower left quadrant (short peak-to-trough time and low peak-to-trough amplitude ratio), borders highlighted with red dotted lines. b. Example average action potential waveforms of a putative fast-spiking cell (black) and a putative pyramidal cell (green). The corresponding cells are marked with asterisks in panel a. c. Average calcium transient waveform for a single action potential in putative interneurons for jGCaMP8f, jGCaMP8m, and jGCaMP8s. Resampling was done with a 20-ms-long mean filter. d. Simultaneous fluorescence dynamics and spikes in jGCaMP8f (top), jGCaMP8m (middle) and jGCaMP8s (bottom) expressing putative interneurons. Fluorescence traces were filtered with a Gaussian filter (σ = 5 ms). e. Zoomed-in view of bursts of action potentials from dotted rectangles in panel d (top, jGCaMP8f; middle, jGCaMP8m; bottom, jGCaMP8s).
