Extended Data Fig. 17: Imaging dendritic spikes in cerebellar Purkinje neurons.
From: Fast and sensitive GCaMP calcium indicators for imaging neural populations
a. Experimental design. Purkinje neurons in cerebellar lobule VI were transduced with a GCaMP variant as in the sample widefield (top right) and 2P (bottom right) images. Dendritic tufts were monitored for complex spike-related activity using 2P microscopy under free-locomotion conditions. b. Sample traces from adjacent dendrites for each variant. c. Half-decay times (Kruskal-Wallis P = 8.66e-11; Dunn’s test P values: 6f to 7f = 0.98, 6f to 8s = 0.99, 6f to 8m = 9.41e-7, 6f to 8f = 1.7e-7, 7f to 8s = 1, 7f to 8m = 0.0041, 7f to 8f = 4.62e-4, 8s to 8m = 0.0021, 8s to 8f = 2.42e-4, 8m to 8f = 0.99). d. Normalized fluorescence traces from the average of 10 events nearest to the median values from each variant. e. Half-rise times (Kruskal-Wallis P = 4.03e-26; Dunn’s test P values: 6f to 7f = 0.0025, 6f to 8s = 1.03e-7, 6f to 8m = 1.81e-10, 6f to 8f = 1.57e-6, 7f to 8s = 0.65, 7f to 8m = 0.10, 7f to 8f = 0.49, 8s to 8m = 0.99, 8s to 8f = 1, 8m to 8f = 1). f. Distribution of ΔF/F0 responses to complex spikes (Kruskal-Wallis P = 2.99e-9; Dunn’s test P values: 6f to 7f = 0.0010, 6f to 8s = 0.013, 6f to 8m = 1.22e-5, 6f to 8f = 0.67, 7f to 8s = 0.99, 7f to 8m = 0.99, 7f to 8f = 3.89e-4, 8s to 8m = 0.83, 8s to 8f = 0.0027, 8m to 8f = 1.40e-5). For each variant, 2 mice were imaged with number of dendrites per variant as: GCaMP6f, n = 51; jGCaMP7f, n = 14; jGCaMP8s, n = 14; jGCaMP8m, n = 13; jGCaMP8f, n = 9. In box plots, boxes indicate median and inter-quartile range (IQR) while whiskers extend to the extrema or 1.5*IQR + (−) q3 (q1) with outliers lying beyond those values.
