Extended Data Fig. 1: Crystal structure of jGCaMP8.410.80.
From: Fast and sensitive GCaMP calcium indicators for imaging neural populations
ENOSP (yellow), linker 1 (ENOSP-cpGFP, grey), linker 2 (cpGFP-CaM, grey), cpGFP (green), CaM (blue), Ca2+ ions (orange). a. Overlay of the structures of jGCaMP8.410.80 and GCaMP5G (light grey). Left: side view. Right: top view. b. A closeup of the chromophore region in structures of jGCaMP8.410.80 and GCaMP5G. Ile32 (dark gray) in Linker 1 of jGCaMP8.410.80 facilitates closer interaction of Tyr352 (blue) with the GFP chromophore. The corresponding residues in GCaMP5G, Glu60 and Tyr380, are depicted in light gray. c. Individual residue mutations screened in this study, shown on the structure of jGCaMP8.410.80. Sixteen initial interface positions are in orange. Ten subsequently mutated CaM positions are in magenta. Mutations based on the FGCaMP sensor are in cyan.
