Extended Data Fig. 3: Whole genome sequencing analysis of mouse tumours.

WGS analysis of tumours from ET mice exposed to air pollution (n = 5) and those exposed to PBS controls (n = 5). Each mouse tumour is compared vs the corresponding germline from the same mouse. A) Mutational profiles for each tumour sample according to the mutation trinucleotide context. LEFT: PBS Controls, RIGHT: 50 μg PM. B) Barplots indicate the counts of mutations in each sample, where bars are colored based on the base change. C) Boxplot comparing the counts of mutations between tumours from pollution exposed mice (50 μg PM) and tumours from PBS exposed mice (PBS Control). All mutations are summarised in one plot on the left, and are then further divided based on the base change of the mutation (n = 5 mice per group). Two-sided t-test comparing numbers of mutations between PBS and air pollution p-values are displayed. The boxplot line represents the median, the hinges of the box represent the 1st and 3rd quartiles and the limits of the whiskers represent the 1.5 interquartile range. D) Attribution of mutations in each tumour sample to each single base substitution (SBS) mutation signature. The shading indicates the weight of the signature within each sample. Majority of the weights have been assigned to ageing related signatures (SBS40, SBS5, SBS1) Komogolomov-Smirnoff test p-value = 0.26–0.68.