Extended Data Fig. 9: DA neuron responses to appetitive stimuli are dependent on Sst+ CeA neurons.
From: Plastic and stimulus-specific coding of salient events in the central amygdala
a, Left: trial-by-trial activities of a water-excited neuron in a mouse receiving water, before (top) and after (bottom) the mouse was treated with DMSO. Right, trial-by-trial activities of another water-excited neuron in a mouse receiving water, before (top) and after (bottom) the mouse was treated with SALB. b, Quantification of the average water-evoked responses of all water-excited DA neurons in individual mice (n = 4 mice; DMSO, n.s., nonsignificant, P = 0.5807; SALB, *P = 0.0212; paired t-test). c & d, same as a & b, respectively, except that shock-excited neurons were analyzed, and mice received shock instead of water (quantification in d: n = 4 mice; DMSO, n.s., P = 0.0794; SALB, n.s., P = 0.3593; paired t-test). e, Left: trial-by-trial (top and middle) and average (bottom) activities of a sucrose-excited neuron in a mouse receiving sucrose, before and after the mouse was treated with DMSO. Right: trial-by-trial (top and middle) and average (bottom) activities of another sucrose-excited neuron in a mouse receiving sucrose, before and after the mouse was treated with SALB. f, Top: Quantification of sucrose-induced responses in DA neurons in one example mouse. All DA neurons showing excitatory responses to sucrose were included (DMSO, n = 16 neurons, n.s., P = 0.8982; SALB, n = 25 neurons, **P = 0.0019; paired t-test). Bottom: quantification of the average sucrose-evoked responses of all sucrose-excited DA neurons in individual mice (n = 4 mice; DMSO, n.s., P = 0.9505; SALB, *P = 0.0132; paired t-test). g & h, same as e & f, respectively, except that quinine-excited neurons were analyzed, and mice received quinine instead of sucrose (quantification in h, top: DMSO, n = 14 neurons, n.s., P = 0.5657; SALB, n = 15 neurons, n.s., P = 0.0984; paired t-test; bottom: n = 4 mice; DMSO, n.s., P = 0.4903; SALB, n.s., P = 0.4020; paired t-test). i & j, same as g & h, respectively, except that quinine-inhibited neurons were analyzed (quantification in j, top: DMSO, n = 3 neurons, n.s., P = 0.7500; SALB, n = 3 neurons, n.s., P = 0.7500; Wilcoxon test; bottom: n = 4 mice; DMSO, n.s., P = 0.5906; SALB, n.s., P = 0.9527; paired t-test). k & l, same as i & j, respectively, except that shock-inhibited neurons were analyzed, and mice received shock instead of quinine (quantification in l, top: DMSO, n = 4 neurons, n.s., P = 0.6961; SALB, n = 4 neurons, n.s., P = 0.3916; paired t-test; bottom: n = 4 mice; DMSO, n.s., P = 0.8263; SALB, n.s., P = 0.2366; paired t-test). Data are presented as mean ± s.e.m. Shaded areas represent s.e.m.
