Extended Data Fig. 5: Enhanced immune cell activation in PPB tumors.
From: PI3Kβ controls immune evasion in PTEN-deficient breast tumours
a–g, Flow cytometry analysis of PP, PPA and PPB tumors harvested five days after tumor cell transplantation into FVB mice (n = 10 tumors per group). Box plots represent median and inter-quartile range, and min-to-max error bars (whiskers). Representative gating profiles to demonstrate that myeloid-derived IFNɣ is primarily produced by inflammatory monocytes are shown on (f). h, PP, PPA and PPB tumor cells were treated with 100 ng/mL IFNɣ in vitro for 48 h. MHC class I (MHC-I) expression was analyzed by flow cytometry (n = 3 biological replicates per condition). Data shown as median and min-to-max error bars. i, PP, PPA and PPB tumor cells were treated with IFNɣ in vitro for 72 h. Cell viability was analyzed by CellTiter-Glo. Data shown as mean values and s.d. (n = 4 biological replicates per condition). For comparison of two means (h), unpaired, two-tail t-test with Welch’s correction assuming unequal variance. For multiple comparisons (a–e, g and i), one-way ANOVA followed by Tukey’s multiple comparisons tests. Flow cytometry gating profiles available on Supplementary Fig. 1.
