Extended Data Fig. 3: Single-cell RNA-Seq analysis of immune infiltrate in PTEN/p53-null breast tumors with catalytic isoform-specific PI3K deletion.
From: PI3Kβ controls immune evasion in PTEN-deficient breast tumours
Single-cell RNA-Seq (scRNA-Seq) analysis of immune infiltrate from PP, PPA and PPB tumors (n = 3 mice per group). Tumors were harvested and dissociated into single cells, followed by FACS sorting for viable immune (CD45+) cells prior to scRNA-Seq analysis via Seq-Well technology. a, Heatmap showing gene expression for top 10% differentially-expressed genes (DEGs) for each cell type cluster. Selected genes for each cluster are indicated on the right. Genotype of the tumor of origin is indicated on top. b, tSNE of all CD45+ cells analyzed denoting experimental replicate and cell type. c, Genotype representation per cell type. Complete list of DEGs per cell type is available in the source data linked to this article.
