Extended Data Table 1 Characterization of Hans- and Mex-LanM metal complexes using SEC-MALS

From: Enhanced rare-earth separation with a metal-sensitive lanmodulin dimer

  1. The concentrations of the protein samples loaded to the column were: 1.2—1.5 mg/mL for apo- and RE-bound Hans-LanM, 3 mg/mL for CaII-bound Hans-LanM, and 3 mg/mL for Mex-LanM. For RE-containing samples, protein was pre-incubated with 3 equiv. of the appropriate REIII ion. In the case of CaII, 5 mM CaCl2 was added to the running buffer. The apoprotein elutes in two peaks, the first being a minor contribution (10% of protein, 56.5 kDa) and the second being the major peak (90% of protein, 12.9 kDa). See Materials and Methods for full details of sample preparation. The values for Hans-LanM are plotted in Fig. 2a; raw data for La, Nd, and Dy are shown in Supplementary Fig. 5.
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