Extended Data Fig. 6: FLIM-FRET measurements of NUP98 inside the NPC show qualitative agreement between live cells and permeabilized cells. | Nature

Extended Data Fig. 6: FLIM-FRET measurements of NUP98 inside the NPC show qualitative agreement between live cells and permeabilized cells.

From: Visualizing the disordered nuclear transport machinery in situ

Extended Data Fig. 6

(a) Fluorescent lifetime curves of double-amber-mutated sample NUP98221TAG-251TAG (Nres = 30 aa) and NUP98221TAG-482TAG (Nres = 261 aa) labelled with donor (JF549) and acceptor dye (JF646) mixture. Each profile represents an averaged result of 6 cells. (b) Fluorescent lifetime curves of double-amber-mutated samples same as in (a) but labelled with donor (AZDye594) and acceptor dye (LD655) in permeabilized cells (same dataset as in Extended Data Fig. 5a for the selected mutants).

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