Extended Data Fig. 8: Single-molecule FRET measurements in solution.
From: Visualizing the disordered nuclear transport machinery in situ
FRET efficiency histograms for different mutants of purified NUP98 FG-domain in 1x PBS. The peaks in the grey shadow indicate the donor-only population. The FRET population was fitted with an asymmetric-Gaussian function to retrieve the FRET efficiency.
