Fig. 2: SARS-CoV-2 virus isolation from environmental samples of the HSM.
From: Surveillance of SARS-CoV-2 at the Huanan Seafood Market
a–d, Electron micrographs of the SARS-CoV-2 viruses isolated from the environmental samples in the HSM. To determine whether SARS-CoV-2 particles could be visualized from the cell supernatant and lysate, we used transmission electron microscopy to observe the culture supernatant and ultrathin sections from Vero E6 and Huh7.5 cells. The electron micrographs showed that virus particles were present in both the supernatant (a,b) and the cells (c,d). Negative-stained virus particles were generally spherical, pleomorphic and 60–140 nm in diameter. Spike protrusions were observed around the particles in a crown (corona) shape (a,b). In ultrathin cultured cell sections, a group of virus particles can be seen outside the cell (c), and sheets of virus particles can also be observed inside the cells (d). The micrographs are representatives of repeated experiments.
