Extended Data Fig. 5: Lineage tracing of the LPM into paired fins and the PAFF.
From: A median fin derived from the lateral plate mesoderm and the origin of paired fins
a–g, Permanently labelled LPM cells can be seen within pectoral and pelvic fins and pre-anal fin fold (arrowheads). Confocal images of the pectoral fin at 4.5 dpf (a), adult pelvic fin (b–d) and pre-anal fin fold at 5 dpf (e–g) of Tg(drl:creERT2); Tg(hsp70l:Switch) transgenics following Hydroxytamoxifen treatment and heat-shock prior to imaging. Pelvic fins and PAFF are shown as transverse sections (b–g) and were fluorescently immunostained for eGFP (b,e), Zns5 (c) or Transgelin (f). Merged images are shown in (d,g). LPM-derived mesenchyme cells of the pectoral and pre-anal fin folds are indicated by white and yellow arrowheads respectively (a, e–g). LPM-derived Zns5-positive osteoblasts of the pelvic fins are indicated by white arrowheads (b–d). Scale Bars: 20 µm (d,g), 50 µm (a).
