Extended Data Fig. 6: Pre-anal fin fold mesenchyme originates from posterior-most drl-positive cells.
From: A median fin derived from the lateral plate mesoderm and the origin of paired fins
a–d, Lateral (a) and ventral (b–d) views of 8ss (a–b) or 10ss (c–d) drl:H2B-Dendra2 transgenic line either prior to (a–b) or following (c–d) photoconversion of posterior-most LPM (region outlined by dotted line) using UV laser illumination. e–p, Lateral confocal images of the PAFF region at 40 hpf (e–g, k–m) and 48 hpf (h–j, n–p) of a photoconverted embryo (e–j) and an unconverted control (k–p). Panels show Dendra2-red (f,i,l,o), Dendra2-green (g,j,m,p) and merged (e,h,k,n) channels. PAFFs are indicated by yellow arrowheads, and are visible in green channel in both converted and controls, but only converted embryos show red labelled PAFFs (f,i; n = 4 photoconverted fish). Magnified views of panels (f,g,i,j,l,m,o,p) are given in panels (q–x) respectively. Scale Bars: 200 µm (b), 50 µm (d,x), 100 µm (k).
