Extended Data Fig. 8: Reduced Chordin leads to paired duplication of PAFF.
From: A median fin derived from the lateral plate mesoderm and the origin of paired fins
a–b, Low (a) and high (b) power Nomarski images of duplicated PAFFs (yellow arrowheads) in 5 dpf larvae injected with chrd morpholino. c–j, Confocal images of PAFFs from 8 dpf Tg(hand2:EGFP) either uninjected (c,e) or injected with chrd morpholino (d,f,g–j). Confocal projections (c,d,i,j) and total surface renderings (e,f,g,h) highlight duplicated PAFFs (blue and yellow, f) compared to single PAFF in uninjected larvae (grey, e). Imaging of left (g) and right (h) duplicated PAFFs of chrd morphants indicated eGFP positive mesenchymal cells populated both fin folds (yellow arrowheads, i,j). k– l, Lateral (k) and ventral (l) Nomarski images of the duplicated PAFFs of the Ranchu goldfish strain at 6 dpf. Duplicated PAFFs and ventral caudal fin folds indicated by yellow and cyan arrowheads, respectively. m–n, Lateral (m) and transverse (n) views of 7 dpf Ranchu larvae stained by in situ hybridisation for hand2. Expression in individual mesenchymal cells of the PAFF indicated by yellow arrowheads (m). Occurrence of three PAFFs with core hand2 expression indicated by yellow arrowheads (n). Scale Bars: 100 µm (a,e,g), 50 µm (b), 500 µm (k), 200 µm (l), 20 µm (m,n).
