Extended Data Fig. 2: Expression of hand2 in the pre-anal fin fold.
From: A median fin derived from the lateral plate mesoderm and the origin of paired fins
a–b, High (a) and low (b) magnification confocal images of Tg(hand2:EGFP) zebrafish embryos at 3 dpf (a) and 8 dpf (b) showing eGFP labelling of mesenchyme of the PAFF (yellow outline, a,b), but not the caudal fin fold (cyan outline, b). c–d, In situ hybridisation of hand2 at 5 dpf showing expression of hand2 only in the PAFF (c). Higher magnification with Nomarski optics indicates expression in mesenchyme (d). Scale Bars: 100 µm (b,c), 50 µm (d), 20 µm (a).
