Fig. 4: Histone modifications modulate OCT4 and SOX2 cooperativity.
From: Histone modifications regulate pioneer transcription factor cooperativity
a, Representative native gel electrophoresis showing OCT4 and SOX2 binding to LIN28B or LIN28B-H3K27ac nucleosomes (left). The coloured asterisks indicate molecules bound to the nucleosome: 1-OCT4 is in red, 1-OCT4 and 1-SOX2 are in orange and 2-OCT4 is in blue. Quantification of SOX2 binding to the OCT4-bound LIN28B-H3K27ac nucleosome relative to OCT4-bound LIN28B (right). Data shown as mean ± s.e.m., n = 4 independent experiments; ***P = 0.0009, one-sided Student’s t-test. b, Representative native gel electrophoresis (n = 2) showing OCT4 and SOX2 binding to LIN28B-H3K27ac nucleosomes and LIN28B-H3K27ac with the SOX2-binding site moved by 5 bp (arrow). The coloured asterisks indicate molecules bound to the nucleosome: the nucleosome is in black, 1-OCT4 is in red, 1-OCT4–SOX2 is in orange, and SOX2 is in grey.
