Fig. 2: Injury repair alters the competitive balance between wild-type and HrasG12V/+ cells in mosaic skin.
From: Injury prevents Ras mutant cell expansion in mosaic skin
a, Schematic of the experimental design using the injury repair model. b, Representative two-photon revisit images of the basal stem cell layer of the epidermis. White lines highlight the boundaries between tdTomato+ and tdTomato− populations. Epithelial nuclei are in green (Krt14-H2B–GFP) and recombined cells are in red (LSL-tdTomato). c, The increase in tdTomato+ area over time in the uninjured condition. n = 3 mice. d, Representative two-photon revisit images of the basal stem cell layer of the epidermis during injury repair. e, The increase in tdTomato+ area over time in the injured condition. n = 4 mice. Unpaired, two-tailed t-test comparing wild-type with HrasG12V/+ mice at different time points in uninjured and injured conditions. P values are shown. At least three independent areas of approximately 300 µm2 were analysed for each mouse (Methods). Data are mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; NS, not significant.
