Fig. 4: KrasG12D/+ cells lose their competitive advantage during injury repair of mosaic skin.
From: Injury prevents Ras mutant cell expansion in mosaic skin
a, Representative two-photon revisit images of the epidermal basal stem cell layer. White lines mark borders between mutant and wild-type cells. b, The increase in tdTomato+ area in uninjured and injured conditions following induction with tamoxifen (uninjured KrasG12D/+ mosaic, n = 4 mice; injured KrasG12D/+ mosaic, n = 5 mice). c, Quantification of mitotic cells in tdTomato+ and tdTomato− areas. Uninjured KrasG12D/+ mosaic, n = 4 mice; injured KrasG12D/+ mosaic, n = 5 mice. d, Heat maps of the top-down (x–y) view of representative two-photon images around the injury at 14 days PWI. Colour represents the thickness of the epithelium. e, Average epithelial thickness at 14 days PWI around the wound in wild-type (n = 3 mice), KrasG12D/+ mosaic (n = 4 mice) and KrasG12D/+ max (n = 3 mice). Solid lines represent means and dashed lines show s.d. b,c, Paired two-tailed t-test comparing tdTomato+ and tdTomato− populations in the same group of mice. Unpaired two-tailed t-test comparing tdTomato+ and tdTomato− populations in different groups of mice and KrasG12D/+ mutant mice in uninjured and injured conditions at different time points. P values are shown. At least three independent areas of approximately 300 µm2 were analysed for each mouse (Methods). Data are mean ± s.d.
