Extended Data Fig. 4: Transcriptome profiling of TAMs and MTMs.
From: Reprogramming tumour-associated macrophages to outcompete cancer cells
a, A schematic diagram showing the Rosa26LSL-MYC allele that encodes human MYC (hMYC) and the internal ribosome entry site (IRES)-driven truncated human CD2 (hCD2) protein as a cell surface reporter. b, Reporter assay showing the specificity of S100a8-cre-mediated recombination of the Rosa26LSL-MYC allele and high reconstitution efficiency of wild-type (WT) bone marrow cells. c, Experimental design of bulk RNA-sequencing experiments for tumor-associated macrophages (TAMs) and mammary tissue macrophages (MTMs) isolated from control-PyMT and MYC-PyMT mice reconstituted with WT bone marrow cells and treated with a low protein (LP) diet (2% protein in weight, 5BT9, TestDiet). d, Flow cytometry gating strategy for TAMs and MTMs. Siglec-F, B220, Ly6G, and Ly6C were used to exclude eosinophils, B cells, neutrophils, and monocytes, respectively. e. PCA analysis of TAMs and MTMs isolated from control-PyMT and MYC-PyMT mice reconstituted with WT bone marrow cells and treated with a LP diet, based on the log2 fold change of gene expression compared to the mean. MTMs from MYC3 group are not included due to insufficient cell number.
