Extended Data Fig. 3: CapZyme-seq analysis of TBEV, BVDV and CHIKV infected cells.
From: Hepatitis C virus RNA is 5′-capped with flavin adenine dinucleotide
Mean-difference plots showing fold changes (log2) as a function of average abundance (log) for reads in 5’ termini of individual RNA molecules. (a) CapZyme-seq analysis of RNA isolated from SH-SY5Y neuroblastoma cells infected with TBEV using Rpp, AtNUDX23, NudC and CapClip for enrichment. NudC has broad specificity, including cleavage of FAD, NAD+ and m7GpppN; Cap-Clip Acid Pyrophosphatase hydrolyzes m7GpppN (n = 3 biological replicates). (b) CapZyme-seq analysis of RNA isolated from MDBK bovine kidney cells infected with BVDV using Rpp and AtNUDX23 for enrichment (n = 3 biological replicates). (c) CapZyme-seq analysis of RNA isolated from TIG3 fibroblast cells infected with CHIKV using Rpp and AtNUDX23 for enrichment (n = 2 biological replicates).
