Extended Data Fig. 2: Ex vivo tetramer and phenotype analysis of NQK-Q8- and NQK-A8-specific T cells, related to Fig. 1d.
From: A common allele of HLA is associated with asymptomatic SARS-CoV-2 infection
(a,b) FACS plots of NQK-A8-tetramer+ CD8+ T cells following tetramer magnetic enrichment (a) and NQK-Q8-tetramer+ CD8+ T cells (b). The NQK-A8- and NQK-Q8- tetramer+ CD8+ T cells shown as blue dots were plotted versus CD3+ T cells shown as grey dots and gated on CCR7 and CD45RA to determine memory status, following tetramer magnetic enrichment for each donor. (c) The percentage of tetramer+ of CD8+ T cells after tetramer magnetic enrichment is reported for each donor with the purple bar for the Tet-Q8 tetramer and the orange bar for the Tet-A8 tetramer (n = 7 and n = 6 biologically independent samples, respectively). Data are presented as median values with IQR (interquartile range). The individual percentage for each donor is indicated on panels a and b. (d) The proportion of Tnaïve (CD45RA+CCR7+CD95-); TSCM (stem cell memory, CD45RA+CCR7+CD95+); TCM (central memory, CD45RA-CCR7+); TEM (effector memory, CD45RA-CCR7-); TEMRA (terminally differentiated, CD45RA+CCR7-) cells in different donors.
