Fig. 1: Cryo-EM structure of Rpd3S complex and interactions among subunits.
From: Diverse modes of H3K36me3-guided nucleosomal deacetylation by Rpd3S
a, Schematic representation of the domain organizations of the Rpd3S complex. The colour scheme for Rpd3S complex subunits is indicated in b. Domains that are not structurally resolved are coloured grey. b, Cryo-EM map of the entire Rpd3S complex integrated with a 2.7 Å map of the head–bridge–right arm region and a 3.2 Å map of the left arm region. WD40, WD40 β-propeller domain. c, Global view of the Rpd3S complex, highlighting the Rco1–Eaf3 heterodimers. The PHD1 (light brown) and MID (reseda) domains of Rco1 and the MRG domain (blue violet) of Eaf3 are shown in surface representation; other domains are shown in cartoon form. The dotted lines represent the SBD surfaces (SBD-R and SBD-L) at different angles. d, Close-up view of interactions of the SBD domains of Rco1 and the PAH3 domain of Sin3 in the SBD-L surface and bridge region. e, Isothermal titration calorimetry fitting curves for indicated histone peptides with PHDs of Rco1. H3un, unmodified H31–10 peptide; PHD1-DM, PHD1 double mutant (E260K/D261K). ND, not determined.
