Extended Data Fig. 7: Cryo-EM structure of Rpd3S complex bound to H3K36me3 nucleosome in the loose state and the interface details between CHD domains and histone H3K36me3.
From: Diverse modes of H3K36me3-guided nucleosomal deacetylation by Rpd3S
a. Core Rpd3S complex bound to the H3K36me3 modified nucleosome in the loose state. b. Stereo view of contact between core Rpd3S complex and H3K36me3 modified nucleosome in the loose state. Sin3, Rpd3, Eaf3, and Rco1 are shown in cartoon form. Nucleosome is shown in surface representation. The invisible left arm region is colored white. c. Detailed view of interactions between CHD and H3 tail. Close-up views of the loop of CHD and H3 tail for interactions with cryo-EM densities shown as meshes. d. Detailed view of the interactions between Eaf3A-CHD and the H3K36me3 modified nucleosome. e. Detailed view of the interactions between Eaf3B-CHD and the H3K36me3 modified nucleosome. The residues of CHDs and H3 tail residues are shown as sticks. The positions of nucleosomal DNA are labeled with SHL numbers.
