Extended Data Fig. 8: The catalytic activity of Rpd3S on modified nucleosomes.
From: Diverse modes of H3K36me3-guided nucleosomal deacetylation by Rpd3S
a. Validation of the site-specificity of antibodies used in HDAC assay. b. A representative HDAC assay measuring activity of Rpd3S complex on H3K4me3K36me3 nucleosome. c. A representative HDAC assay measuring activity of Rpd3S complex on H3K9RK36me3 nucleosome. A representative HDAC assay measuring the activity of Rpd3S complex containing wild-type (d), mutants of PHD1 (e), and left arm region (f) on H3K36me3 and H3K36me0 nucleosomes. The reaction products were identified using Western blot. One representative example of three (a-f) independent experiments is shown.
