Fig. 5: In vivo modification crosstalk studies.
From: Diverse modes of H3K36me3-guided nucleosomal deacetylation by Rpd3S
a, The test cryptic transcription phenotype caused by Rco1(E260K/D261K) or Rco1(7mu) mutants in a STE11-HIS3 reporter strain (YBL853). WT, wild type. b, Western blot showing H3 and H4 acetylation levels at different sites in Rco1 wild type, E260K/D261K and 7mu mutants. c, Growth of H3 wild type and H3K9R mutant yeast. Fivefold serial dilutions of H3 wild type and H3K9R mutant cells were spotted onto plates with synthetic complete medium (with glucose) containing 100 μg ml−1 6-azauracil (6-AU) and cultivated at 30 °C for 3 days. d, Western blot showing global H3 and H4 acetylation defects in the H3K9R mutant yeast strain. a–d, One representative example of three independent experiments. e,f, Models of the Rpd3S complex bound to the H3K36me3 modified nucleosome on histone H4 deacetylation (e) and on histone H3 deacetylation (f).
