Extended Data Fig. 1: Protein purification and XL-MS analysis of Rpd3S complex.
From: Diverse modes of H3K36me3-guided nucleosomal deacetylation by Rpd3S
a-c. Purification of Rpd3S and mutant complexes. Purification of the Rpd3S and mutant complexes. The complexes were purified using size-exclusion chromatography (Superose 6), and the peak fractions were subjected to SDS-PAGE for Coomassie blue staining. The complexes include: (a) wild-type of Rpd3S complex; (b) Rco1-E260A/D261A of the Rpd3S complex to disrupt the PHD1 of Rco1; (c) Rco1-7mutants of Rpd3S complex to disrupt the left arm region. d. Schematic representation of the intermolecular cross-links within the Rpd3S complex. The domains of Rpd3S are indicated, and the identified inter-subunit cross-links or subunit self-links are shown as cyan or modena solid lines, respectively. The special intermolecular self-links in the C-terminal of Rco1 are shown as red solid lines.
