Extended Data Fig. 10: GDF15 blockade reduces allergen avoidance.
From: Immune sensing of food allergens promotes avoidance behaviour
a, Cumulative licks of OVA over time and (b) total licks of OVA and water in mast cell-depleted RMB mice retreated with rGDF15 (mg/kg) from one experiment pertaining to Fig. 4e (n = 3 WT control, 5 WT allergic, 4 MC Dep, 6 MC Dep + 0.001mg/kg, 6 MC Dep + 0.01 mg/kg, 7 MC Dep + 0.1 mg/kg rGDF15). c, BALB/c mice were sensitized with OVA/alum and treated with a mouse GDF15 blocking antibody or isotype control 5 h prior to two bottle preference testing. d, Total IgE (left), OVA-specific IgE (centre), and OVA-specific IgG1 (right) antibodies after neutralizing GDF15 antibody treatment (n = 11 isotype, 10 aGDF15). e, Cumulative licks during the two-bottle preference test of isotype control (left) or GDF15 blocking antibody-treated (right) mice (n = 6 isotype, 6 aGDF15). f, OVA preference of OVA/alum sensitized mice that received either GDF15 blocking antibody or isotype control 5 h prior to the behavioural assay (n = 6 isotype, 4 aGDF15). Graphs show mean ± s.e.m. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. a-b, One-way ANOVA with Dunnett’s multiple comparisons test, d, Two-tailed Student’s t-test, f, Two-tailed Mann-Whitney test. Each panel is representative of at least two independent experiments. c, Created with BioRender.com.
