Fig. 2: Allergic avoidance requires IgE and mast cells.
From: Immune sensing of food allergens promotes avoidance behaviour
a, Total (left) and OVA-specific (right) levels of serum IgE on day 14 after allergic sensitization in BALB/c mice (n = 6 control and 11 allergic mice per group). b, Cumulative licks to water and OVA solutions during the two-bottle preference test in OVA + alum-sensitized IgE-KO (right) and wild-type (WT) littermate control (left) mice (n = 6 WT and 6 IgE-KO mice per group). c, Drinking preference to OVA bottles in controls and allergic sensitized WT or IgE-KO mice (n = 9–11 mice per group). d, Cumulative licks to water and OVA bottles in OVA + alum-sensitized WT (left) or FCER1 (right) chimaeras. WT or FCER1-KO bone marrow haematopoietic cells were transplanted into irradiated WT recipients (n = 5 WT to FCER1 and 6 FCER1 into WT). e, Drinking preference to OVA bottles in allergic sensitized WT or FCER1 chimaeras (n = 9 WT to FCER1 and 7 FCER1 into WT). f, OVA-specific IgE (n = 11 WT to FCER1 and 9 FCER1 into WT). g, Schematic protocol of FCER1+ cell depletion with diphtheria toxin (DT) in RMB BALB/c mice. h, Cumulative licks to water and OVA bottles in allergic sensitized and diphtheria toxin-injected RMB WT (left) and RMB mutants (right) (n = 9 RMB WT and 14 RMB heterozygotes or mutants). MC ∅, mast cell depleted. i,j, Preference to OVA solution (n = 8 RMB WT and 12 RMB heterozygotes or mutants) (i) and OVA-specific IgE (n = 6 per group) (j) in diphtheria toxin-injected RMB WT and mutants. Graphs show mean ± s.e.m. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001; NS, not significant. Two-tailed Mann–Whitney test. Each panel is representative of at least two independent experiments. g, Created with BioRender.com.
